TRACKING SYSTEM FOR ALTERNATIVE METHODS TOWARDS REGULATORY ACCEPTANCE
TSAR indicates the stages methods have reached in terms of acceptance as a recognised test method for use in various sectors together with a summary description. Where available, TSAR also includes relevant records and documents associated with a method linked to the different steps of the entire process: submission, validation, peer-review, recommendations and regulatory acceptance including international standards represented in the tracking system.

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Combined assay for the detection of tetanus toxicity in tetanus toxoids
TM Status: Closed
Test Method Number: TM2012-01 (EU)
Short Name of TM: Tettox
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Quality control of vaccines
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    Submission
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    Validation
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    Peer-review
  •  
    Recommendation
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    Regulatory acceptance/Standards
In vitro Caco-2 permeability
TM Status: Closed
Test Method Number: TM2013-01 (EU)
Short Name of TM: IVCP assay
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Intestinal permeability/penetration, toxicokinetics,
Toxicokinetics
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
3T3 Neutral Red Uptake cytotoxicity assay
TM Status: Closed
Test Method Number: TM2007-03 (EU)
Short Name of TM: 3T3 NRU
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Acute toxicity,
Basal Cytotoxicity
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
Neutral red uptake for starting doses for acute oral toxicity
TM Status: Closed
Test Method Number: TM2003-01 (US)
Short Name of TM: NRU cytotoxicity
Responsible Organisation: ICCVAM/NICEATM - United States 
Topic(s): Acute toxicity,
Basal Cytotoxicity
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    Submission
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    Validation
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    Peer-review
  •  
    Recommendation
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    Regulatory acceptance/Standards
KeratinoSens assay for the testing of skin sensitizers
TM Status: Closed
Test Method Number: TM2010-03 (EU)
Short Name of TM: KeratinoSens
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Skin sensitisation
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    Submission
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    Validation
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    Peer-review
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    Recommendation
  •  
    Regulatory acceptance/Standards