TRACKING SYSTEM FOR ALTERNATIVE METHODS TOWARDS REGULATORY ACCEPTANCE
TSAR indicates the stages methods have reached in terms of acceptance as a recognised test method for use in various sectors together with a summary description. Where available, TSAR also includes relevant records and documents associated with a method linked to the different steps of the entire process: submission, validation, peer-review, recommendations and regulatory acceptance including international standards represented in the tracking system.

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3D Reconstructed Human Skin Comet assay
TM Status: Open
Test Method Number: TM2020-01 (EU)
Short Name of TM: RS Comet assay
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Genotoxicity/Mutagenicity
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    Submission
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    Validation
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    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
3D Reconstructed Human Skin Micronucleus assay (RSMN)
TM Status: Open
Test Method Number: TM2020-04 (EU)
Short Name of TM: RSMN
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Genotoxicity/Mutagenicity
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    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
Cytochrome P450 (CYP) enzyme induction in vitro method using cryopreserved differentiated human HepaRG™ cells
TM Status: Open
Test Method Number: TM2009-14 (EU)
Short Name of TM: HepaRG™
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Toxicokinetics
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    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
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    Regulatory acceptance/Standards
In Vitro Skin Sensitisation: The FBS free ARE-Nrf2 Luciferase α-Sens® Test Method
TM Status: Open
Test Method Number: TM2022-03 (JAP)
Short Name of TM: α-Sens®
Responsible Organisation: JaCVAM - Japan
Topic(s): Skin sensitisation
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    Submission
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    Validation
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    Peer-review
  •  
    Recommendation
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    Regulatory acceptance/Standards
Lung Surfactant Bioassay
TM Status: Open
Test Method Number: TM2026-01 (EU)
Short Name of TM: LS Bioassay
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Acute toxicity,
Human Health Effects
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    Submission
  •  
    Validation
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    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards