TRACKING SYSTEM FOR ALTERNATIVE METHODS TOWARDS REGULATORY ACCEPTANCE
TSAR indicates the stages methods have reached in terms of acceptance as a recognised test method for use in various sectors together with a summary description. Where available, TSAR also includes relevant records and documents associated with a method linked to the different steps of the entire process: submission, validation, peer-review, recommendations and regulatory acceptance including international standards represented in the tracking system.

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3D Reconstructed Human Skin Comet assay
TM Status: Open
Test Method Number: TM2020-01 (EU)
Short Name of TM: RS Comet assay
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Genotoxicity/Mutagenicity
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
Blood-brain barrier-on-a-chip (BBB-oC) for drug testing of brain-targeting delivery
TM Status: Closed
Test Method Number: TM2024-01 (EU)
Short Name of TM: BBB-oC
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Human health
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
Bioaccessibility testing (Bioelution) of metals, inorganic metals compounds and metals-containing materials: simulated gastric fluid
TM Status: Open
Test Method Number: TM2016-02 (EU)
Short Name of TM: Bioelution
Responsible Organisation: EURL ECVAM - European Union 
Topic(s): Bioaccessibility
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
Epidermal Sensitization Assay
TM Status: Closed
Test Method Number: TM2018-01 (JAP)
Short Name of TM: EpiSensA
Responsible Organisation: JaCVAM - Japan
Topic(s): Skin sensitisation
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards
Androgen Receptor Transactivation Assay for Detection of androgenic Agonist and Antagonist Activity of Chemicals using the stably transfected human 22Rv1/MMTV_GR-KO cell line
TM Status: Open
Test Method Number: TM2020-02 (OECD)
Short Name of TM: 22Rv1/MMTV_GR-KO
Responsible Organisation: OECD - International Organisation
Topic(s): Endocrine disruption
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    Submission
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    Validation
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    Peer-review
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    Recommendation
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    Regulatory acceptance/Standards