Topic: Skin sensitisation
Test Method Number:
TM2022-01 (EU)
Short Name of TM:
ProtReact
Year received:
2022
Responsible Organisation:
General Comments:
Method Description
The ProtReact is an in chemico method that addresses the covalent binding of electrophilic chemicals with nucleophilic sites of amino acids in skin proteins (i.e., haptenation) which represents the molecular initiating event of the skin sensitisation Adverse Outcome Pathway.
The test method measures reactivity of a test chemical by quantifying through fluorescence/absorbance, the depletion of lysine and cysteine peptides in 96 well-plates.
It is proposed to contribute to the skin sensitisation hazard assessment of chemicals.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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