3D Reconstructed Human Skin Micronucleus assay (RSMN)

Method Description

The RS MN Assay is an in vitro genotoxicity assay, which transfers the known micronucleus test in a three-dimensional reconstructed human skin (3D RHS) model. The assay addresses the potential of test items to cause genotoxicity in the form of chromosomal damage (clastogenicity and aneugenicity). The method uses the EpiDerm™ Skin Model (MatTek, Ashland, USA) which is cultured from normal human epidermal keratinocytes derived from neo-natal foreskin tissue on specifically prepared tissue culture inserts. As such, the method is aimed to predict the potential of substances that are primarily associated with dermal exposure to cause DNA damage.