Topic: Endocrine disruption
Test Method Number:
TM2020-02 (OECD)
Short Name of TM:
22Rv1/MMTV_GR-KO
Year received:
2020
Responsible Organisation:
OECD - International Organisation
Method Description
The 22Rv1/MMTV_GR-KO AR-mediated stably transfected transcriptional activation (TA) assay was established to screen chemicals for endocrine activity via interaction with the AR using a human prostate cancer cell line, 22Rv1, that endogenously expresses the AR (1, 2). This test method is specifically designed to detect human AR-mediated TA and inhibition by measuring luciferase activity as the endpoint. The information of chemical dependent interference with luminescence signals is limited in a GR-knockout 22Rv1/MMTV cell line.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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