3D Reconstructed Human Skin Comet assay

Method Description

The RS Comet Assay is an in vitro genotoxicity assay, presented as an adaptation of the known alkaline comet assay and designed in a three-dimensional reconstructed human skin (RHS) model. The method uses specifically the Phenion® Full-Thickness Skin Model which is composed of two tissues from primary and p53 competent cells of human origin, primary keratinocytes and fibroblasts. As such, the method is aimed to predict the potential of substances that are primarily associated with dermal exposure to cause DNA damage.