Topic: Skin sensitisation
Test Method Number:
TM2018-01 (JAP)
Short Name of TM:
EpiSensA
Year received:
2018
Responsible Organisation:
JaCVAM - Japan
Protocol(s)/SOP(s):
General Comments:
Method Description
The EpiSensA is an in vitro method for measuring the expression of four marker genes (ATF3, encoding Activating transcription factor 3; GCLM, encoding Glutamate-cysteine ligase, modifier subunit; DNAJB4, encoding DnaJ (Hsp40) homolog, subfamily B, member4; IL-8, encoding Interleukin-8) of the reconstructed human epidermis (RhE) following controlled exposure of test chemicals. The two main important keratinocyte responses that occur in the skin sensitization adverse outcome pathway are Inflammatory response and Induction of cytoprotective gene pathways. The assay is based on the induction of the multiple marker genes related to these two keratinocyte responses.
Track Approval Status
-
Submission
-
Validation
-
Peer-review
-
Recommendation
-
Regulatory acceptance/Standards
| Step | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Submission | Show status | ||||||||
|
|||||||||
| Validation | Show status | ||||||||
|
|||||||||
| Peer-review | Show status | ||||||||
|
|||||||||
| Recommendation | Show status | ||||||||
|
|||||||||
| Regulatory acceptance/Standards | Show status | ||||||||
|
|||||||||