|Test Method Number:||TM2016-09 (US)|
|Short Name of TM:||VM7Luc ER TA|
|Responsible Organisation:||ICCVAM/NICEATM - United States '|
|General Comments:||This test method was originally referred to as the BG1Luc ER TA method.|
The VM7Luc4E2 cell line was derived from the MCF7 human breast cancer cell line that endogenously express both human ER forms, ERα and ERβ, and that have been stably transfected with the plasmid pGudLuc7.ERE. This plasmid contains four copies of a synthetic oligonucleotide containing the estrogen response element upstream of the mouse mammary tumor viral promoter and the firefly luciferase gene. VM7Luc4E2 cells emit measurable light in response to estrogen and estrogen-like substances.
In 2016, NICEATM received new information regarding the LUMI-CELL® test. DNA testing has now...
|Regulatory acceptance/Standards||Show status|