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The The VM7Luc ER TA test method: An In Vitro Assay for Identifying Human Estrogen Receptor Agonist and Antagonist Activity of Chemicals

Topic : Reproductive toxicity, Endocrine disruption

Test Method Number: TM2016-09 (US)
Short Name of TM: VM7Luc ER TA
Year received: 2016
Responsible Organisation: ICCVAM/NICEATM - United States '
Protocol(s)/SOP(s): 455
General Comments: This test method was originally referred to as the BG1Luc ER TA method.

Method Description

The VM7Luc ER TA test method (also known as the LUMI-CELL® test method) was developed by Xenobiotic Detection Systems, Inc., with support from NIEHS Small Business Innovation Research grant ES10533-03. The test method measures the extent to which a substance induces or inhibits transcriptional activity (TA) via ER-mediated pathways in recombinant VM7Luc4E2 cells.

The VM7Luc4E2 cell line was derived from the MCF7 human breast cancer cell line that endogenously express both human ER forms, ERα and ERβ, and that have been stably transfected with the plasmid pGudLuc7.ERE. This plasmid contains four copies of a synthetic oligonucleotide containing the estrogen response element upstream of the mouse mammary tumor viral promoter and the firefly luciferase gene. VM7Luc4E2 cells emit measurable light in response to estrogen and estrogen-like substances.

In 2016, NICEATM received new information regarding the LUMI-CELL® test. DNA testing has now...

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Track Approval Status

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  • Validation
  • Peer-review
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  • Regulatory acceptance/Standards
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