Hen’s Egg Test – Chorio-Allantoic Membrane

Topic: Serious eye damage/Eye irritation

Test Method Number:
TM2016-05 (BRA)
Short Name of TM:
HET-CAM
Year received:
2016
Responsible Organisation:

Method Description

This method is intented to enter a battery of assays to replace rabbits on Eye Irritation Test, as indication of non-irritant substances and a Bottom-Up strategy. Leghorn SPF eggs are used. The test substance is applied to the surface of the CAM. The CAM is evaluated by the appearance of following: congestion (hyperemia), hemorrhage (bleeding), and coagulation (intra- and extravascular). In Brazil, HET-CAM is already accepted by ANVISA as a screening test, but nor for regulatory purposes.

Track Approval Status

  •  
    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
Step
Submission
Validation
Peer-review
Recommendation
Regulatory acceptance/Standards