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SkinEthic™ HCE Eye Irritation Test

Topic : Serious eye damage/Eye irritation

Test Method Number: TM2015-03 (EU)
Short Name of TM: SkinEthic HCE EIT
Year received: 2015
Responsible Organisation: EURL ECVAM - European Union '
General Comments:

External validation performed. Report submitted for peer-review as full test submission.

Preceding TM: TM2008-09 - Human Reconstructed Corneal Epithelium (HCE) Eye Irritation test

Method Description

The SkinEthic™ Human Corneal Epithelium (HCE) Eye Irritation Test (EIT) method is an in vitro assay used for assessing eye irritation/serious eye damage. The method uses a reconstructed human cornea-like epithelium  (RhCE) consisting of a multilayered epithelium prepared from immortalized human corneal epithelial cells which reproduce the multi-layer organization of the human cornea. The tissues are exposed to the test chemicals according to two possible protocols, depending on the physical state of the test chemical either solid "EITS" protocol (see DB-ALM protocol No.191), or liquid/viscous "EITL" protocol ( see DB-ALM protocol No.190).

Track Approval Status

  • Submission
  • Validation
  • Peer-review
  • Recommendation
  • Regulatory acceptance/Standards
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