Skin Irritation Test for the Prediction of Acute Skin Irritation of Chemicals With Sterlab RHE: 42 minutes application + 42 hours post-incubation

The present test method is designed to predict and classify the skin irritant potential of chemicals according to chemical safety regulation, using STERLAB human reconstructed epidermis (RHE) model. The protocol is established for liquids, viscous and solid test substances. The relative viability of the treated tissues is measured at the end of the treatment exposure (42 minutes) followed by a post-exposure period (42 hours) using a cell viability test.