Topic: Skin irritation, Skin sensitisation, Genotoxicity/Mutagenicity
Test Method Number:
TM2013-04 (JAP)
Short Name of TM:
Ros Assay
Year received:
2013
Responsible Organisation:
JaCVAM - Japan
General Comments:
Method Description
Phototoxicity is defined as a toxic response is elicited by topically or systemically administered photoreactive chemicals after the exposure of the body to environmental light. The ROS assay was designed for qualitative photoreactivity assessment of chemicals, the principle of which is monitoring of type I (an electron or hydrogen transfer, resulting in the formation of free radical species) and type II (an energy transfer from excited triplet photosensitizer to the oxygen) photochemical reactions in test chemicals exposed to simulated sunlight. In the ROS assay, SO generation is detected by spectrophotometric measurement of p-nitrosodimethylaniline (RNO) bleaching, followed by decreased absorbance of RNO at 440 nm.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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