Genomic Embryonic Stem cell Test method

Method Description

The method addresses to test human embryotoxicity and consequently predicts developmental effects on the basis of alterations of gene expression of cell differentiation. The method has showed a large potential discriminating weak embryotoxicants form non-embryotoxicants without loss of capability to detect strong embryotoxicants.

The test method compares the relationships among alterations of gene expression in D3 cells and changes on viability on the same D3 mouse embryonic stem cells and 3T3 mouse using as parameters alterations on cell viability determined by the MTT test and alterations on gene expression determined by quantitative RT-PCR.