Transcriptomics based Genomics-Genotox assay

Topic: Genotoxicity/Mutagenicity

Test Method Number:
TM2010-08 (EU)
Short Name of TM:
GGA
Year received:
2010
Responsible Organisation:
General Comments:
Does not qualify for entering validation

Method Description

The Genomics-genotox assay is a transcriptomics-based in vitro test performed in HepG2 cells to accurately predict the in vivo genotoxic properties of chemicals. Mechanistic relevance of the classifier genes is represented by the fact that many of those genes are regulated by relevant transcription factors, such as the DNA damage response-related SP1 gene for the Ames-positive classifiers, and the involvement in stress response pathways for the Ames-negative classifiers.

Stratification of the compounds based on the Ames- test, further adds mechanistic relevance, as it grossly separates compounds that are DNA-reactive (either direct or after metabolic activation) from those that are not reactive.

Track Approval Status

  •  
    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
Step
Submission Show status
Validation
Peer-review
Recommendation
Regulatory acceptance/Standards