|Test Method Number:||TM2009-05 (EU)|
|Short Name of TM:||MUSST|
|Responsible Organisation:||EURL ECVAM - European Union '|
Validation stopped. New submission with new protocol received under TM2013-02 (U-Sens)
|Succeeding TM:||TM2013-02 - U-SENS™ - Myeloid U937 Skin Sensitisation Test|
The U937 cells are human myeloid cells used as surrogate model for DCs. U937 is a cell line established from a diffuse histiocytic lymphoma with the advantage of higher stability and the easiness of maintenance compared to human-blood derived DCs. The CD86 protein marker is a costimulatory factor which is upregulated in mature DCs and plays a pivotal role in antigen presentation and subsequent T cell priming. In the MUSST test method, the modulation of the FITC-labeled anti-CD86 in U937 cells is measured by flow cytometry after a 48 hours exposure to test chemicals.