Valitox an alternative in vitro toxicity assay

Topic: Acute toxicity

Test Method Number:

TM2008-12 (EU)

Short Name of TM:

Valitox

Year received:

2008

Responsible Organisation:

EURL ECVAM - European Union

General Comments:

This method was later officially submitted as DAP (Dequenching after photobleaching cytotoxicity test). See TM2011-01

Method Description

Fluorescent method that allows for analysis of cell death through plasma membrane permeation detection. The cell impermeant SYTOX DNA binding dye was used. In dying or dead cells the dye crosses the plasma membrane, reaches the nucleus and binds DNA leading to an increase of fuorescent signal. The concentration of a chemical that is lethal to 50% of HeLa cells (LC50) is calculated. The ultimate aim is to predict acute human toxicity.

This method was later officially submitted as DAP (Dequenching after photobleaching cytotoxicity test). See TM2011-01

Track Approval Status

Submission
Validation
Peer-review
Recommendation
Regulatory acceptance/Standards

Submission

Show status

Date	Stage	Comment
Dec 2008	Receipt of presubmission	The report of a study performed between March and July 2008 was received. The EURL ECVAM submission form was not used.
Dec 2008	Assessment of presubmission	Outcome of the assessment of the information provided in the report was communicated. Test submitter was asked to clarify a number of issues and to fill in the EURL ECVAM submission form
Apr 2011	Stopped	Submission closed because replaced by TM2011-01

Validation

Peer-review

Recommendation

Regulatory acceptance/Standards