Topic: Skin sensitisation
Method Description
The CPA-I test aims to identify the proteins associated with type I (IgE mediated) hypersensitivity reactions. The immune response in hypersensitivity reactions towards proteins are mainly of IgE/Th2 cell type (i.e. the type I hypersensitivity reaction). The CPA-I exploits inherent characteristics of proteins, which enable them to polarize the subsequent immune response- as demonstrated by the production of neopterin, a specific immune modulator.
The test utilizes a human myleomonocytic cell line, that is cultivated for 48 hrs in the presence of serial dilutions (within the range of non- toxic concentrations) of the test substance. Supernatants are collected and the concentration of neopterin is measured using ELISA. For each substance, a dose response curve is established. The cells are cultivated in serum free media. Cell viability is determined by propidium iodide (PI). The potential allergens to be tested can be tested as component of extracts or in...
The CPA-I test aims to identify the proteins associated with type I (IgE mediated) hypersensitivity reactions. The immune response in hypersensitivity reactions towards proteins are mainly of IgE/Th2 cell type (i.e. the type I hypersensitivity reaction). The CPA-I exploits inherent characteristics of proteins, which enable them to polarize the subsequent immune response- as demonstrated by the production of neopterin, a specific immune modulator.
The test utilizes a human myleomonocytic cell line, that is cultivated for 48 hrs in the presence of serial dilutions (within the range of non- toxic concentrations) of the test substance. Supernatants are collected and the concentration of neopterin is measured using ELISA. For each substance, a dose response curve is established. The cells are cultivated in serum free media. Cell viability is determined by propidium iodide (PI). The potential allergens to be tested can be tested as component of extracts or in pure native form.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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