The h-CLAT supports the discrimination between sensitizing and non-sensitizing chemicals
The h-CLAT quantifies in vitro changes in the expression of the CD86 and CD54 membrane phenotypic markers in a human monocytic leukemia cell line (THP-1 cells).
THP-1 cells are monocyte-derived cells that have shown to produce DC-like responses following exposure to skin sensitising chemicals (e.g. upregulation of surface markes (e.g. CD86 and CD54) and cytokine production (e.g. TNF-α).
The CD86 (a co-stimulatory molecule) and the CD54 (an adhesion molecule) are upregulated in activated Dendritic Cells (DC) and play a critical role in DC presentation of antigens to T cells (T-cell priming).
By studying the potential of test chemicals to up-regulate markers of DC activation, the h-CLAT generates information addressing key event 3 (dendritic cell activation) of the skin sensitization Adverse Outcome Pathway.