Topic: Fish acute toxicity
Test Method Number:
TM2006-01 (EU)
Short Name of TM:
ZFET
Year received:
2006
Responsible Organisation:
Protocol(s)/SOP(s):
Method Description
The zebrafish embryo acute toxicity test method (ZFET) is carried out with newly fertilised eggs from zebrafish (Danio rerio). It is an acute test that uses short-term exposure (96 hours) and determines the concentration of a chemical that is lethal to 50% of zebrafish embryos (LC50) as a prediction of acute fish toxicity. The following observations indicate death of an embryo: coagulation of the embryo, lack of somite formation, non-detachment of the tail and/or lack of heartbeat.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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