Zebrafish embryo acute toxicity test for acute fish toxicity testing

Topic: Fish acute toxicity

Test Method Number:
TM2006-01 (EU)
Short Name of TM:
ZFET
Year received:
2006
Responsible Organisation:
Protocol(s)/SOP(s):
140

Method Description

The zebrafish embryo acute toxicity test method (ZFET) is carried out with newly fertilised eggs from zebrafish (Danio rerio). It is an acute test that uses short-term exposure (96 hours) and determines the concentration of a chemical that is lethal to 50% of zebrafish embryos (LC50) as a prediction of acute fish toxicity. The following observations indicate death of an embryo: coagulation of the embryo, lack of somite formation, non-detachment of the tail and/or lack of heartbeat.

Track Approval Status

  •  
    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
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