Topic: Endocrine disruption
Test Method Number:
TM2019-13 (EU)
Short Name of TM:
MCT8-TR
Year received:
2019
Responsible Organisation:
General Comments:
Method Description
This method measures the active transport of thyroid hormone T3 across the plasma membrane by monocarboxylate transporter 8 (MCT8).
T3 uptake, by Madin Darby Canine Kidney (MDCK) cells stably transfected with human MCT8, is measured with a specrophotometric absorbance readout following the Sandell-Kolthoff (SK) reaction, during which the yellow cerium(IV) is reduced to the colourless cerium (III).
This method was selected with other methods to assess modulating effects on the thyroid system (OECD, 2014). Information on these selected methods is available in TSAR as ‘Thyroid methods’.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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