|Test Method Number:||TM2014-06 (EU)|
|Short Name of TM:||Sterlab RHE SIT|
|Submission received on:||October, 2014|
|Responsible Organisation:||EURL ECVAM - European Union|
|Stage of Submission:||Assessment Finalised|
Further clarifications requested
The relative viability of the treated tissues is measured at the end of the treatment exposure (42 minutes) followed by a post-exposure period (42 hours) using a cell viability test.
A cut-off value of 50% viability of the negative control value is used to classify test substances as irritant (I) or non-irritant (NI).
Cell viability is measured by dehydrogenase conversion of MTT, present in cell mitochondria, into a blue formazan salt that is quantitatively measured after extraction from tissues. The reduction of viability of tissues exposed to chemicals in comparison to negative controls is used to predict skin irritation potency. The quantitative...