|Test Method Number:||TM2013-02 (EU)|
|Short Name of TM:||U-SENS™|
|Submission received on:||June, 2013|
|Responsible Organisation:||EURL ECVAM - European Union|
|Stage of Submission:||Assessment Finalised|
|Stage of Validation:||Finalised|
|Stage of Peer-review:||Finalised|
The U-SENS™ test method was formely called Myeloid U937 Skin Sensitisation Test (MUSST) (TM 2009-05).
|Preceding TM:||TM2009-05 - Myeloid U937 Skin Sensitisation Test|
The U937 cells are human myeloid cells used as surrogate model for DCs. U937 is a cell line established from a diffuse histiocytic lymphoma with the advantage of higher stability and the easiness of maintenance compared to human-blood derived DCs. Although being of monocytic origin, U937 cells have shown DC-like responses following exposure to sensitizers including the up-regulation of surface marker (e.g. CD86) and cytokine production (e.g. TNF-a). The up-regulation of CD86 plays important roles in the induction of Allergic Contact Dermatitis (ACD). The CD86 protein marker is a costimulatory factor which is upregulated in...