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Hen’s Egg Test – Chorio-Allantoic Membrane

Topic : Eye irritation

Test Method Number: TM2016-05 (BRA)
Short Name of TM: HET-CAM
Submission received on: November, 2016
Responsible Organisation: BraCVAM - Brazil

Method Description

This method is intented to enter a battery of assays to replace rabbits on Eye Irritation Test, as indication of non-irritant substances and a Bottom-Up strategy. Leghorn SPF eggs are used. The test substance is applied to the surface of the CAM. The CAM is evaluated by the appearance of following: congestion (hyperemia), hemorrhage (bleeding), and coagulation (intra- and extravascular). In Brazil, HET-CAM is already accepted by ANVISA as a screening test, but nor for regulatory purposes.

Track Approval Status

  • 1
    Submission
  • 2
    Validation
  • 3
    Peer-review
  • 4
    Recommendation
  • 5
    Regulatory acceptance/Standards
Step
 Submission 
 Validation 
 Peer-review 
 Recommendation 
 Regulatory acceptance/Standards 
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