Topic: Skin sensitisation
Test Method Number:
TM2016-10 (JAP)
Short Name of TM:
IL-8 Luciferase assay for skin sensitization
Year received:
2016
Responsible Organisation:
JaCVAM - Japan
General Comments:
Method Description
The IL-8 Luc assay measures changes in IL-8 expression, a cytokine associated with the activation of dendritic cells (key event 3) for evaluating skin sensitisation potential of chemicals. In the THP-1-derived IL-8 reporter cell line, IL-8 expression is measured as luciferase activity.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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