Multiplexed SRM (Selected Reaction Monitoring) Mass Spectrometric Assays for Targeted Quantification of Skin Sensitization Biomarkers in Dendritic Cell Models

Topic: Sensitisation

Test Method Number:
TM2011-08 (EU)
Short Name of TM:
DC-SRM
Year received:
2011
Responsible Organisation:
EURL ECVAM - European Union 
General Comments:
On hold-pending further information

Method Description

Multiplexed SRM (Selected Reaction Monitoring) is a Mass Spectrometric Assays for Targeted Quantification of Skin Sensitization Biomarkers in Dendritic Cell (DC) Models. The test is designed for dichotomous classification (Yes/No).

The method comprises steps of contacting a relevant DC model, preferentially MUTZ-3 progenitor cells, with potential sensitizers, non-sensitizers and solvent controls, and measuring the change in protein expression using targeted mass spectrometric assays. MUTZ-3 are a human myelomonocytic cell line which was identified as the human dendritic cell (DC)-like cell line that is closest to human monocyte derived DC.

The protein biomarker model for skin sensitization is based on detailed proteomic analysis of relevant cell lines. A panel of 15 most promising biomarkers allows discriminating between sensitizers and non-sensitizers. Additional 10 biomarkers allow to identify respiratory sensitizers in MUTZ-3 cells

Track Approval Status

  •  
    Submission
  •  
    Validation
  •  
    Peer-review
  •  
    Recommendation
  •  
    Regulatory acceptance/Standards
Step
Submission Show status
Validation
Peer-review
Recommendation
Regulatory acceptance/Standards