Topic: Acute toxicity
Test Method Number:
TM2008-12 (EU)
Short Name of TM:
Valitox
Year received:
2008
Responsible Organisation:
General Comments:
This method was later officially submitted as DAP (Dequenching after photobleaching cytotoxicity test). See TM2011-01
Method Description
Fluorescent method that allows for analysis of cell death through plasma membrane permeation detection. The cell impermeant SYTOX DNA binding dye was used. In dying or dead cells the dye crosses the plasma membrane, reaches the nucleus and binds DNA leading to an increase of fuorescent signal. The concentration of a chemical that is lethal to 50% of HeLa cells (LC50) is calculated. The ultimate aim is to predict acute human toxicity.
This method was later officially submitted as DAP (Dequenching after photobleaching cytotoxicity test). See TM2011-01
Track Approval Status
-
Submission
-
Validation
-
Peer-review
-
Recommendation
-
Regulatory acceptance/Standards
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