|Test Method Number:||TM2009-09 (EU)|
|Short Name of TM:||EST-1000 / epiCS|
|Submission received on:||Tuesday, November 3, 2009|
|Responsible Organisation:||EURL ECVAM - European Union|
|Stage of Submission:||Assessment of Full submission|
|Stage of Validation:||Finalised|
|Stage of Peer-review:||Finalised|
|General Comments:||ESAC peer review requested additional testing|
The test system is composed of Reconstructed human Epidermis (RhE) tissues. This model constitutes a three dimensional structure which reproduces the typical multi-layer cell organization of the epidermis. The tissues are exposed to the test chemicals during 20 minutes. This is followed by a post-exposure period of 24±2 hours in order to derive the cell viability of the tissues.
Mitochondrial dehydrogenases of living cells are able to reduce the MTT reagent into blue formazan. The Sterlab RHE SIT protocol measures the optical density (OD) of the blue formazan in the treated tissues. The OD is normalised to the one of the negative control in order to express cell viability values in percentages.
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