Topic: Acute toxicity
Test Method Number:
TM2014-05 (EU)
Short Name of TM:
LUCS
Year received:
2014
Responsible Organisation:
General Comments:
Not prioritised for validation by ECVAM since it has not fulfilled ECVAM requirements.
Succeeding TM:
Method Description
In vitro test method designed to predict and classify acute and geno-toxicity.
The test method measures nucleic acid alteration in live cells through fluorescence readout in 96- or 384-well plates. The underlying cellular mechanism involves DNA and/or RNA damage by Reactive Oxygen Species (ROS). It takes advantage of the ROS-induced variations in fluorescence intensities in cells incubated with a membrane-permeant nucleic acid sensor of the cyanine asymmetrical family.
It is proposed as a replacement of the 3T3-NRU assay in all its applications including phototoxicity and applicable also to skin corrosion and irritation regulatory testing under REACH cosmetics and biocides.
Track Approval Status
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Submission
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Validation
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Peer-review
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Recommendation
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Regulatory acceptance/Standards
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