TSAR (Tracking System for Alternative test methods Review Validation and Approval in the Context of EU Regulations on Chemicals).

TSAR : Tracking System for Alternative test methods Review, Validation and Approval in the Context of EU Regulations on Chemicals

Eye Irritation

Fluorescein Leakage Test (FL) - INVITTOX Protocol 71

3 Rs Category	:	Partial Replacement
Type of Method	:	In vitro
Validation Statement	:	Scientific validity of test method (ESAC): 10/07/2009.
Comment	:	Useful for the identification of ocular corrosives and severe irritants only. If combined with other validated alternative test methods in the frame of a testing strategy (e.g. Bottom-Up and Top-Down Approach, Scott L. et al. 2009), could eventually lead to the complete replacement of the classical in vivo Draize eye test.
Status of adoption in EU	:	Not included in EU regulation.
Corresponding OECD TG	:	None
Status of adoption by OECD	:	Not included and not foreseen yet. No OECD activity.